Coding
GB1

Part:BBa_K3308055

Designed by: Jemy Varghese, Ripal Sheth, Victor So, Harrison Green, Melissa Marciesky   Group: iGEM19_Pittsburgh   (2019-10-05)


Immunoglobulin-binding protein G domain B1 (GB1) extein- SICST-GB1+ stop

We decided that Immunoglobulin-binding protein G domain B1 (GB1) would be a viable extein sequence, utlizing it's well characterize structure and previous usage as an extein in intein research. This part includes the flanking sequence used so that splicing of IMPDH-1 at the c-terminus cna be optimized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None